Download PDF by Barbara J. Bain: Blood Cells: A Practical Guide

By Barbara J. Bain

  • Enables either the haematologist and laboratory scientist to spot blood telephone positive aspects, from the commonest to the extra obscure
  • Provides crucial details on equipment of assortment, blood movie instruction and marking, including the foundations of guide and automatic blood counts
  • Completely revised and up to date, incorporating a lot newly released info: now contains suggestion on extra checks while a particular prognosis is suspected
  • 400 prime quality photos to assist with blood mobilephone identification
  • Highlights the aim and scientific relevance of haematology laboratory checks throughout

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Extra resources for Blood Cells: A Practical Guide

Example text

Inaccuracy can also be introduced into a count if many smear cells (see Chapter 3) are present and are not included in the count. If the smear cells are, for example, lymphocytes, then the percentage and absolute number of lymphocytes will be falsely low and the percentage and absolute number of all other cell types will be falsely high. Smear cells whose nature can be deduced can be counted with the category to which they belong. Otherwise smear cells and any other unidentifiable cells, if present in significant numbers, should be counted as a separate category or the percentage and absolute number of cells of all categories will be erroneous.

01 l/l of the reference PCV. The red blood cell count (RBC), more usually referred to as the red cell count, was initially performed by count­ ing red cells microscopically in a carefully diluted sample of blood contained in a counting chamber (haemocyto­ meter) with chambers of known volume [21]. Although this method was capable of producing satisfactory results if great care was exercised, it proved very unreliable in routine use because of a high degree of imprecision, and it was also very time‐consuming.

Three simultaneous measurements are made on each cell: (i) impedance measurements with low‐frequency electromagnetic current, dependent mainly on cell volume; (ii) conductivity measurements with high‐ frequency (radiofrequency) electromagnetic current, which alters the bipolar lipid layer of the cell membrane allowing the current to penetrate the cells and is therefore dependent mainly on the internal structure of the cell, including nucleocytoplasmic ratio, nuclear density and granularity; (iii) forward light scattering at 10–70° when cells pass through a laser beam determined by the structure, shape and reflectivity of the cell.

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